Genetic Reprogramming Of The Production Of The Valuable Chemical In Plants Research Report

Project

Your company grows plants that produce a valuable chemical X in floral petals. Genes responsible for the biosynthesis of this chemical are GeneXA, GeneXB and GeneXC. These genes are regulated by the petal-specific transcription factor, TFX.

Problem: These plants cannot be commercially used for the large-scale production of X because:

they do not produce many flowers (Figure 1) flowers are small and the production is seasonal and short since these plants flower only in spring and flowering lasts just a few days.

As a result, it is not economical to produce enough biomass of petals for the commercial production of chemical X.

Your project

To increase the production of the chemical X your company decided to reprogram the expression of genes XA, XB and XC, re-directing the production of chemical X from flowers to leaves of the same plants. The rationales behind this decision are:

• these plants have big leaves,
• leaves growth is not seasonal and
• leaves and flowers have the same genome, and all genes involved in X production exist in leaves, but are not active.

You are the lucky researcher who must run this project. You need to design a strategy to conduct this project.

Research task:

• Design and describe a transgene for the production of chemical X in leaves (eg promoter, coding region, terminator). You can design more than one transgene. Show transgene(s) as a diagram/map: 
• Design and describe a vector that contains your transgene(s). Show vector as a map: 
• Design a strategy for transformation, generation, and selection of the transgenic plants: 
• Design strategies to assess and confirm your generated transgenic plant: 

Your report:

You can add pictures and diagrams if required. Use paragraphs to separate different parts of the review. Show in bold critical components of the gene silencing mechanisms.

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