BUIRET SPECTROPHOTOMETRY TEST - AUDIT TASKS Weeks 5-8
BUIRET SPECTROPHOTOMETRY TEST - AUDIT TASKS Weeks 5-8
Instructions
Download VALID TEST REPORTS-alphabetical v2.docx (from Canvas) These are the test reports for tests done in June and July
Download WORKSHEETS Biuret v2.docx for the same 2 sets of patients (from Canvas)
Download the Audit Biuret Test Reports versus Worksheets Template v2.xlsx excel file (from Canvas)
Name the excel file so that it includes June-July and your first and last name.
Follow the below S.O.P. to audit the 2 sets of test results and associated technical records.
Record results of the audit in the audit template excel file.
Submit your excel file with the completed task(s) for that week to the link in Assignments in Canvas. There will be a separate link for each week.
WEEK 5
Part 1: Verify accuracy of data and technical records
Part A. Worksheet and Test Report Comparison
Part B. LIMS data and worksheet data comparison
Due this week:
Submit Audit Biuret Test Reports versus Worksheets Template v2.xlsx with Part 1A and Part 1b tabs completed
WEEK 6
Part 2: Assess quality of data/results
Part 3: Review Calibration and Equipment Logs - Start Part 3 if time permits
Due this week:
Submit Audit Biuret Test Reports versus Worksheets Template v2.xlsx with Part 2-LJ Control Charts tab completed
WEEK 7
Complete Part 3, Respond to feedback on Part 1&2
Part 3: Review Calibration and Equipment Logs
Part 3A. - Calibration Log
Part 3B. - Equipment Log
Due this week:
Submit Audit Biuret Test Reports versus Worksheets Template v2.xlsx with Part 3A and 3B calibration log tabs completed
Submit responses to feedback - for Parts 1 & 2
WEEK 8
Due this week:
This is the last session of the course
Last chance to complete & submit all assessment tasks
All sections must be completed correctly to achieve satisfactory for this assessment.
S.O.P. Audit of Test results and associated technical documents
Version 1.0
Release date: 4-8-2020
Written by: Dr P. Athologista
Authorised by: L. Abmanager
Part 1: Verify accuracy of data and technical records
Worksheet and Test Report Comparison
GOAL: Compare information on Test Reports to original data on Worksheets
Retrieve the worksheets for June (Set 1) and July (Set 2) Biuret Tests and their test reports (these are described in Instructions on p1).
Collate the Test Reports (currently filed alphabetically) so that they are in the same order as the worksheets (filed by date). Submit the Collated Reports to the WEEK 5 Collated Test Reports Assignment link in Canvas.
In the Audit template excel file go to the tab called Part 1A-Checklist & Summary
For all Valid Reports in June and July:Check the following values have been correctly transferred to each Test Report and record your findings for each Test Report in the excel checklist.
Control concentration value is actually in the valid range
Control is marked valid on the Test Report
The Control result (concentration in g/L) on the report is the same as written on the worksheet
The Patient result (concentration of protein in g/L) on the Test Report is the same as written on the worksheet
Count the number of each type of non conformance, and summarise in the yellow cells in column L of the excel file (Part 1A-Checklist & Summary tab).
B. LIMS data and worksheet data comparison
GOAL: Compare LIMS entries to original data on Worksheets
Complete the Checklist in the Part 1b LIMS v worksheets tab in the Audit template excel file for the below tasks summarising your findings as you go.
You have been allocated 4 Lab Barcodes to Audit (2 from June and 2 from July). Get these from the teacher.
For the 4 barcodes allocated to you find the relevant Worksheets.
Check each of the 4 worksheets to see whether:
Absorbance data entry on the worksheet matches the LIMS results entries (for all 4 patients in the June and July reports)Identify results in LIMS that do not reconcile with the worksheet (technical record) by colouring the cell(s) in the LIMS yellow.Review Standard, Control and Patient data. Record yes/no for each in excel file tab called Part 1b LIMS v worksheets
Check worksheets and LIMS entries to see whether alterations to data are made clearly, and original information is still viewableRecord findings in the excel checklist (Part 1b Columns AE to AP).
Check excel calculation answers for whether the calculations are correct (compare to excel answers to the calculations) and shown clearlyRecord any non-conformances in the checklist. Give correct answer to calculations where required.
Part 2: Assess quality of data/results
On the excel spreadsheet tab Part 2 LJ Control Charts
Collate control results from all worksheets into 2 separate tables (one for June data and one for July data)The data you need to include is:
the date of the test and
the control protein concentration
Calculate the mean and SD for each of (a) June and (b) July control results.
Calculate the mean +/- 1 and 2SD ranges for each data set.
Plot a separate Levey Jennings chart for each month (Use Line chart in excel). On the charts also show the mean and +/- 1SD and +/-2SD.
Determine the systematic uncertainty (bias) present (if any) for each month compared to the manufacturers information. Do this by comparing each mean you have calculated to the manufacturers mean (equation is given below).The manufacturers published range for the control is: mean 55 g/L, 2SD range: 50-60 g/L
Bias = observed mean - manufacturers mean (Note whether the value is negative or positive)
Calculate the Measurement Uncertainty (MU) for each months data set.
Where MU = 2SD (Use the SD values you calculated for each dataset ie one for June data, and another for July data)
How does the MU for each month that you calculated compare to that expected? TIP: Find the expected MU given with the patient value on each test report.
Part 3: Review Calibration and Equipment Logs
GOAL
Review the calibration log and equipment log (user checks) for accuracy and completeness.
A. Calibration Log
The data for this task is provided in the Audit Template on the Part 3a - Calibration Log tab.
Compare the data from June only in the calibration log for the spectrophotometer to the worksheet data. Conditional formatting has been set up to find data outside of acceptable ranges.
Identify affected Test Results by using date and standard information. Record the barcode of all affected patients (June only results are required) in Column I of the spreadsheet.
What action/s need to be taken for each of these non-conformances? Record these actions in column J of the spreadsheet.Choose from: annotate worksheet with non-con / unpublish test result / retest sample
Hint: Consider what effects on test results this non-conformance will have Will the results still be reliable? If not, what records needs to be altered? Does anything else need to be done?
B. Equipment Log
Review the equipment log in the Part 3b Equipment Log tab of Audit Template.xlsx. Conditional formatting has been set up to highlight any no responses.
Review the comments/corrective actions (Columns G & N), and Highlight in yellow any issues that might indicate the instrument is not meeting test specifications (i.e. where the reported issue may cast doubt on whether the instrument is working correctly).
In column Q of this tab, record whether the instrument meets test specifications or not.Record as yes = there were no issues recorded, instrument was working ok OR no = the issue may impact test results (further validation/verification required)
Which Test Result/s may be affected by the issue/s identified in the equipment log?Report the lab barcode of any affected patient reports in Column R of the Part 3b tab
Rubric
All parts of the assessment must be completed to satisfactory standard to pass this assessment task.
Part 1: Verify accuracy of data and technical records Satisfactory Not Satisfactory
Test reports collated by order of date Excel file:
Filename follows required naming convention
Worksheet and Test Report ComparisonA completed & correct Checklist that shows results of audit review for worksheet and test report comparison of 2 sets of data (June, July)
LIMS data and worksheet data comparisonAbsorbance data in LIMS highlighted yellow where LIMS data are non-conforming with worksheet results
Checklist that summarises findings of Audit review of worksheet/LIMS data non-conformances is complete and correct Part 2: Assess quality of data/results All required Data collated as described in the SOP
Levey-Jennings Charts for (a) June data, and (b) July data shown clearly labelled fully (title, axes labelled with units, includes on each chart the mean +/-1SD and +/-2SD values
Bias recorded and calculations shown clearly
MU recorded and calculations shown clearly Part 3: Review Calibration and Equipment Logs Calibration log reviewed for accuracy and completeness
Results recorded in the excel checklist as required in the SOP
Checklist is accurate and complete Equipment log (user checks) reviewed for accuracy and completeness
Results recorded in the excel checklist as required in the SOP
Checklist is accurate and complete
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Rosalyn A. Rubin
389128013391100* Patient UR: 653-500036
* Address: 760 Clark Street, Dunlop, VIC
* D.O.B: 05/07/1985
* Requesting Dr: Dr Morris Dawson
* Date of Request: 02/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 56 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 54 +/- 5.5 g/L Reference Interval: 60-80 g/L. LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 2/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 2/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Polly E. Bijur37953959207500* Patient UR: 725-602513
* Address: 160 Elizabeth Street, Melbourne, VIC
* D.O.B: 22/09/1968
* Requesting Dr: Dr Ross Cooper
* Date of Request: 02/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 56 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 46 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 2/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 2/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Jean Golding
376301013557300* Patient UR: 653-500041
* Address: 396 Clarendon St, South Melbourne, VIC
* D.O.B: 20/11/1958
* Requesting Dr: Dr Liam Cooper
* Date of Request: 03/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 52 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 59 +/- 5.5 g/L Reference Interval: 60-80 g/L BORDERLINE LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 3/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 3/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Emily A. Troyer
37260318968400* Patient UR: 645-051440
* Address: 22 Forsyth street, Melrose, VIC
* D.O.B: 18/03/1975
* Requesting Dr: Dr Stuart Jones
* Date of Request: 05/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 52 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 53 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 5/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 5/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Jordan N. Kohn
369211413133300* Patient UR: 889-663476
* Address: 9 Merry Street, Sunshine, VIC
* D.O.B: 09/11/1997
* Requesting Dr: Dr Clarke Smith
* Date of Request: 06/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 53 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 58 +/- 5.5 g/L Reference Interval: 60-80 g/L BORDERLINE LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 6/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 6/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Sarah H. Sell
389064516446500* Patient UR: 479-5546466
* Address: 22 Duncan Street, Glenroy, VIC
* D.O.B: 10/02/1999
* Requesting Dr: Dr Ivan Press
* Date of Request: 07/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 50 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 47 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 7/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 7/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Abraham Eisen
377507518415000* Patient UR: 767-6766757
* Address: 463 Victoria St, West Melbourne, VIC
* D.O.B: 01/12/1954
* Requesting Dr: Dr Donna Copeland
* Date of Request 08/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 55 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 54 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: I. Techo Irma Techo. Date: 8/6/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR P. ATHOLOGISTA P. Athaologista Date: 8/6/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Suzi Hong
400050015557500* Patient UR: 450-986540
* Address: 36 Licosau Avenue, Footscray, VIC
* D.O.B: 15/04/1993
* Requesting Dr: Dr John Small
* Date of Request: 02/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 56 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 47 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo. Date: 2/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 2/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Cynthia Rosenblatt
422910015557500* Patient UR: 320-951325
* Address: 122 Albert St, Port Melbourne, VIC
* D.O.B: 15/02/1982
* Requesting Dr: Dr Thomas Shirin
* Date of Request: 03/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 52 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 50 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature B. Techo Betty Techo.. Date: 3/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 3/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Susan Rose
400050015557500* Patient UR: 125-653970
* Address: 22 Carrie street, Bruce, VIC
* D.O.B: 15/08/1989
* Requesting Dr: Dr John Smith
* Date of Request: 03/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 52 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 60 +/- 5.5 g/L Reference Interval: 60-80 g/L BORDERLINE NORMAL
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo.. Date: 3/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 3/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Emanuel O. Doyne40005004127500* Patient UR: 663-989457
* Address: 12 Seychelle street, Coombs, VIC
* D.O.B: 29/12/1993
* Requesting Dr: Dr Kerry Langan* Date of Request: 05/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 52 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 48 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo.. Date: 5/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 5/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Emanuel O. Doyne400050015557500* Patient UR: 663-989457
* Address: 12 Seychelle street, Coombs, VIC
* D.O.B: 29/12/1993
* Requesting Dr: Dr Kerry Langan* Date of Request: 06/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
FORMCHECKBOX Spectrophotometry:(specify wavelength): _____
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 53 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 48 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo.. Date: 6/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 6/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Mary Haslum422910015557500* Patient UR: 225-4646447
* Address: 60 Frank Street, Richmond, VIC
* D.O.B: 20/09/1969
* Requesting Dr: Dr Maria Ramsay
* Date of Request: 07/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 50 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: _50 +/- 5.5 g/L Reference Interval: 60-80 g/L LOW
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo.. Date: 7/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 7/7/2020
Laboratory Test Request Form & Results Report
* All fields marked with an asterisk must be completed, otherwise delays may occur.
SECTION A
348615065405Laboratory no.(barcode)
00Laboratory no.(barcode)
* Patient name: Edmond P. Zimsky411416515557500* Patient UR: 484-7776658
* Address: 90 Collins St, Melbourne, VIC
* D.O.B: 10/07/1968
* Requesting Dr: Dr Catherine Peckham
* Date of Request: 08/06/2020
SECTION B
* Clinical details:
FORMCHECKBOX Fasting FORMCHECKBOX OCP FORMCHECKBOX HRT FORMCHECKBOX Pregnant - Gestation: ___________
High blood pressure.
Kidney function?
SECTION C
* Test(s) Requested:
FORMCHECKBOX FBE FORMCHECKBOX LFT FORMCHECKBOX LIP FORMCHECKBOX Glucose FORMCHECKBOX Total protein (serum) FORMCHECKBOX Platelets
FORMCHECKBOX Iron studies FORMCHECKBOX U&E FORMCHECKBOX ALP FORMCHECKBOX Other:________________________________
SECTION D
* Specimen type:
FORMCHECKBOX Blood - Whole FORMCHECKBOX Blood - Serum FORMCHECKBOX Blood - plasma FORMCHECKBOX Urine FORMCHECKBOX Swab
SECTION E
* Results:
Control:VALID RANGE: 50-60 g/L
FORMCHECKBOX Valid Value: 55 g/L
FORMCHECKBOX InvalidValue: _______________________________ FORMCHECKBOX Retest required
Patient value: 60 +/- 5.5 g/L Reference Interval: 60-80 g/L BORDERLINE NORMAL
Results entered in LIMS: FORMCHECKBOX
TECHNICIAN name and signature: B. Techo Betty Techo.. Date: 8/7/2020
RESULTS Report approved for release by (SUPERVISOR name and signature):
DR H. Aematol H. Aematol Date: 8/7/2020
Worksheet Biuret Total ProteinDate of analysis: 02-Jun-20
Barcode no. (Patient 1): 881163 Patient 2: 881164Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.962 0.961 n/a n/a n/a
0.960 3a & 3b
Control serum
0.455 0.449 = 0.449 * 1.2 0.961
= 0.56 100 56
0.442 4a & 4b
Patient serum 1
881163 0.437 0.435 = 0.435 * 1.2 0.961
= 0.543 100 54
0.433 5a & 5b
Patient serum 2
881164 0.437 0.440 = 0.440 * 1.2 0.961
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 02-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 02-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 02-Jun-20
Barcode no. (Patient 1): 881567 Patient 2: 881568Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.963 0.965 n/a n/a n/a
0.967 3a & 3b
Control serum
0.469 0.450 = 0.450 * 1.2 0.965
= 0.56 100 56
0.431 4a & 4b
Patient serum 1
881567 0.368 0.370 = 0.370 * 1.2 0.965
= 0.460 100 46
0.372 5a & 5b
Patient serum 2
881568 0.437 0.440 = 0.440 * 1.2 0.965
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 02-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 02-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 03-Jun-20
Barcode no. (Patient 1): 983561 Patient 2: 983562Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.959 0.954 n/a n/a n/a
0.949 3a & 3b
Control serum
0.420 0.413 = 0.413 * 1.2 0.954
= 0.52 100 52
0.407 4a & 4b
Patient serum 1
983561 0.475 0.468 = 0.468 * 1.2 0.954
= 0.598 0.589 100 59
0.461 5a & 5b
Patient serum 2
983562 0.437 0.440 = 0.440 * 1.2 0.954
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 03-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 03-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 05-Jun-20
Barcode no. (Patient 1): 776231 Patient 2: 776232Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.957 0.954 n/a n/a n/a
0.951 3a & 3b
Control serum
0.404 0.414 = 0.414 * 1.2 0.954
= 0.52 100 52
0.424 4a & 4b
Patient serum 1
776231 0.422 0.424 = 0.424 * 1.2 0.954
= 0.533 100 53
0.426 5a & 5b
Patient serum 2
776232 0.437 0.440 = 0.440 * 1.2 0.954
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 05-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 05-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 06-Jun-20
Barcode no. (Patient 1): 724896 Patient 2: 724897Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.963 0.965 n/a n/a n/a
0.967 3a & 3b
Control serum
0.415 0.426 = 0.426 * 1.2 0.965
= 0.53 100 53
0.437 4a & 4b
Patient serum 1
724896 0.475 0.470 = 0.470 * 1.2 0.965
= 0.584 100 58
0.465 5a & 5b
Patient serum 2
724897 0.437 0.440 = 0.440 * 1.2 0.965
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 06-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 06-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 07-Jun-20
Barcode no. (Patient 1): 512136 Patient 2: 512137Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.969 0.964 n/a n/a n/a
0.958 3a & 3b
Control serum
0.408 0.402 = 0.402 * 1.2 0.964
= 0.50 100 50
0.395 4a & 4b
Patient serum 1
512136 0.375 0.381 = 0.381 * 1.2 0.964
=0.454 = 0.474 100 47
0.386 5a & 5b
Patient serum 2
512137 0.437 0.440 = 0.440 * 1.2 0.964
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 07-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 07-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 08-Jun-20
Barcode no. (Patient 1): 986711 Patient 2: 986712Analysed by: Irma Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: P.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.955 0.959 n/a n/a n/a
0.963 3a & 3b
Control serum
0.442 0.440 = 0.440 * 1.2 0.959
= 0.55 100 55
0.437 4a & 4b
Patient serum 1
986711 0.438 0.432 = 0.432 * 1.2 0.959
= 0.541 100 54
0.426 5a & 5b
Patient serum 2
986712 0.437 0.440 = 0.440 * 1.2 0.959
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Irma Techo Irma Techo 08-Jun-20
Reviewed & Approved by (supervisor): P. Athologista P. Athologista 08-Jun-20
Worksheet Biuret Total ProteinDate of analysis: 02-Jul-20
Barcode no. (Patient 1): 542712 Patient 2: 542713Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.952 0.955 n/a n/a n/a
0.958 3a & 3b
Control serum
0.446 0.446 = 0.446 * 1.2 0.955
= 0.56 100 56
0.445 4a & 4b
Patient serum 1
542712 0.375 0.378 = 0.378 * 1.2 0.955
= 0.475 100 47
0.381 5a & 5b
Patient serum 2
542713 0.437 0.440 = 0.440 * 1.2 0.955
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 02-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 02-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 03-Jul-20
Barcode no. (Patient 1): 542648 Patient 2: 542649Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.951 0.953 n/a n/a n/a
0.955 3a & 3b
Control serum
0.432 0.413 = 0.413 * 1.2 0.953
= 0.52 100 52
0.393 4a & 4b
Patient serum 1
542648 0.397 0.401 = 0.401 * 1.2 0.953
= 0.414 100 41
0.404 5a & 5b
Patient serum 2
542649 0.437 0.440 = 0.440 * 1.2 0.953
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 03-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 03-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 04-Jul-20
Barcode no. (Patient 1): 542637 Patient 2: 542638Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.960 0.961 n/a n/a n/a
0.962 3a & 3b
Control serum
0.405 0.416 = 0.416 * 1.2 0.961
= 0.52 100 52
0.427 4a & 4b
Patient serum 1
542637 0.476 0.483 = 0.483 * 1.2 0.961
= 0.602 100 60
0.489 5a & 5b
Patient serum 2
542638 0.437 0.440 = 0.440 * 1.2 0.961
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 04-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 04-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 05-Jul-20
Barcode no. (Patient 1): 542596 Patient 2: 542597Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.966 0.962 n/a n/a n/a
0.958 3a & 3b
Control serum
0.410 0.418 = 0.418 * 1.2 0.962
= 0.52 100 52
0.425 4a & 4b
Patient serum 1
542596 0.381 0.385 = 0.385 * 1.2 0.962
= 0.480 100 48
0.389 5a & 5b
Patient serum 2
542597 0.437 0.440 = 0.440 * 1.2 0.962
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 05-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 05-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 06-Jul-20
Barcode no. (Patient 1): 542410 Patient 2: 542411Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.956 0.958 n/a n/a n/a
0.960 3a & 3b
Control serum
0.422 0.423 = 0.423 * 1.2 0.958
= 0.53 100 53
0.424 4a & 4b
Patient serum 1
542410 0.394 0.387 = 0.387 * 1.2 0.958
= 0.485 100 48
0.380 5a & 5b
Patient serum 2
542411 0.437 0.440 = 0.440 * 1.2 0.958
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 06-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 06-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 07-Jul-20
Barcode no. (Patient 1): 542422 Patient 2: 542423Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.967 0.964 n/a n/a n/a
0.961 3a & 3b
Control serum
0.408 0.402 = 0.402 * 1.2 0.964
= 0.50 100 50
0.395 4a & 4b
Patient serum 1
542422 0.403 0.402 = 0.402 * 1.2 0.964
= 0.500 100 50
0.401 5a & 5b
Patient serum 2
542423 0.437 0.440 = 0.440 * 1.2 0.964
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 07-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 07-Jul-20
Worksheet Biuret Total ProteinDate of analysis: 08-Jul-20
Barcode no. Patient 1: 542415 Patient 2: 553348Analysed by: Betty Techo
Table 1: Addition of Samples and Reagents for Biuret Protein Determination
Tube Description Protein (mL) 1. Protein (L) Add first Water (mL) 2. Water (L) Add second Biuret reagent (mL) 3. Biuret (L) Add last!
1 Blank 0 0 mL 1.0 0 mL 4.0 4.0 mL
2a & 2b
Protein Standard 0.5mL BSA (12g/L*) 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
3a & 3b
Control serum 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
4a & 4b
Patient serum 1 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
5a & 5b
Patient serum 2 0.5mL of 1/10 dilution 0.5 mL 0.5 0.5 mL 4.0
4.0 mL
*Note the std conc is different from the SOP value (10mg/mL) Authorized change: H.A________
Table 2 Absorbance measurements of samples, control and standard and calculations
Tube Description Abs@540 nm Average Abs Diluted conc (g/L) Dilution factor Concentration of original g/L(undiluted)
Using calculation method
1
Blank
0.000 n/a n/a n/a 0
2a & 2b
Protein Standard
12 g/L 0.962 0.963 n/a n/a n/a
0.964 3a & 3b
Control serum
0.448 0.441 = 0.441 * 1.2 0.963
= 0.55 100 55
0.434 4a & 4b
Patient serum 1
542415 0.480 0.484 = 0.484 * 1.2 0.963
= 0.703 100 70
0.488 5a & 5b
Patient serum 2
553348 0.437 0.440 = 0.440 * 1.2 0.963
= 0.550 100 55
0.442 Data processed and checked by technician performing analysis:
Betty Techo Betty Techo 08-Jul-20
Reviewed & Approved by (supervisor): H. Aematol H. Aematol 08-Jul-20
